Biology › Section H · Gene expression and its regulation › Topic H.3

Eukaryotic transcription and RNA processing

Prokaryotes transcribe with one polymerase and translate immediately; eukaryotes keep their genes behind a nuclear membrane, split the work across three polymerases, and put every raw transcript through finishing school - caps, tails, and splicing - before it ever meets a ribosome.

In this topic

  • Same process, new address
  • The three RNA polymerases
  • The mushroom poison test
  • A bigger, busier promoter
  • Transcription factors build the platform
  • Elongation through chromatin, and three endings
  • Finishing school: cap and tail
  • Splicing out the interruptions
  • Worked example: poison as a diagnostic
  • Worked example: the off-by-one splice
  • Common mistakes to avoid
  • Key takeaways

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